1LJI

CRYSTAL STRUCTURE OF MONOCLINIC LYSOZYME GROWN IN PRESENCE 10% SORBITOL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	27.783, 62.707, 60.285
Unit cell angles	90.00, 90.73, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.182
Rwork	0.182
R-free	0.23200
RMSD bond length	0.005
RMSD bond angle	23.400 *
Data reduction software	AUTOMAR
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000	2.000 *
Rmerge	0.075 *	0.165 *
Total number of observations	61435 *
Number of reflections	13571 *	1290 *
Completeness [%]	96.4 *	94.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	293	Sodium Acetate (0.1M) Buffer, Sodium Nitrate (0.44M) Precipitant, pH 4.6, LIQUID DIFFUSION at 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium acetate	0.1 (M)	pH4.6
2	1	1	sorbitol	10 (%)