1LJF

CRYSTAL STRUCTURE OF MONOCLINIC LYSOZYME GROWN IN PRESENCE OF 10% SUCROSE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	27.994, 62.916, 60.398
Unit cell angles	90.00, 90.64, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.197
Rwork	0.197
R-free	0.22700
RMSD bond length	0.005
RMSD bond angle	23.400 *
Data scaling software	SCALEPACK
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.054 *	0.124 *
Total number of observations	135208 *
Number of reflections	18248 *	1789 *
Completeness [%]	93.4 *	90.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	293	Sodium Acetate (0.1M) - Buffer, Sodium Nitrate (0.44M) - Precipitant, Sucrose (0.29M) - Additive; pH 4.6, LIQUID DIFFUSION at 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium acetate	0.1 (M)	pH4.6
2	1	1	sucrose	10 (%)