1LJE

CRYSTAL STRUCTURE OF MONOCLINIC LYSOZYME GROWN IN PRESENCE OF 10% SUCROSE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	28.005, 62.912, 60.476
Unit cell angles	90.00, 90.64, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.173
Rwork	0.173
R-free	0.21200
RMSD bond length	0.005
RMSD bond angle	23.100 *
Data scaling software	SCALEPACK
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.046 *	0.087 *
Total number of observations	47985 *
Number of reflections	14182 *	1392 *
Completeness [%]	99.3 *	99.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	293	Sodium Acetate (0.1M) - Buffer, Sodium Nitrate (0.44M) - Precipitant, Sucrose (0.29M) - Additive, pH 4.6, LIQUID DIFFUSION at 298K, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium acetate	0.1 (M)	pH4.6
2	1	1	sucrose	10 (%)