1LJ3

CRYSTAL STRUCTURE OF MONOCLINIC LYSOZYME GROWN AT PH 4.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	27.886, 62.760, 60.273
Unit cell angles	90.00, 90.91, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.178
Rwork	0.178
R-free	0.23300
RMSD bond length	0.005
RMSD bond angle	23.200 *
Data reduction software	AUTOMAR
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.052 *	0.161 *
Total number of observations	39179 *
Number of reflections	13653 *	1311 *
Completeness [%]	96.7 *	95.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	293 *	Sodium Acetate (0.1M) Buffer, Sodium Nitrate (0.44M) Precipitant, pH 4.6, LIQUID DIFFUSION at 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium acetate	0.1 (M)	pH4.6