1LJ2
Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2001-08-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0447 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.690, 74.040, 77.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.380 |
Rwork | 0.221 |
R-free | 0.27200 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.010 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.460 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.074 * | 0.316 * |
Total number of observations | 110178 * | |
Number of reflections | 26797 * | |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 * | PEG-MME 550, glucose, theophylline, unbuffered, VAPOR DIFFUSION, HANGING DROP at 275K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG550 MME | 17 (%) | |
2 | 1 | reservoir | glucose | 2.5 (%(w/v)) | |
3 | 1 | reservoir | theophylline | 1.5 (mM) | |
4 | 1 | drop | protein | 7 (mg/ml) |