1LJ2

Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	93
Detector technology	CCD
Collection date	2001-08-01
Detector	MARRESEARCH
Wavelength(s)	1.0447
Spacegroup name	P 21 21 21
Unit cell lengths	59.690, 74.040, 77.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.380
Rwork	0.221
R-free	0.27200
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.010
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.460
High resolution limit [Å]	2.380	2.380
Rmerge	0.074 *	0.316 *
Total number of observations	110178 *
Number of reflections	26797 *
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		4 *	PEG-MME 550, glucose, theophylline, unbuffered, VAPOR DIFFUSION, HANGING DROP at 275K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG550 MME	17 (%)
2	1	reservoir	glucose	2.5 (%(w/v))
3	1	reservoir	theophylline	1.5 (mM)
4	1	drop	protein	7 (mg/ml)