1LIS

THE CRYSTAL STRUCTURE OF A FERTILIZATION PROTEIN

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	52.600, 45.900, 81.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.187
Rwork	0.187
RMSD bond length	0.016
RMSD bond angle	2.850
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.070 *
Number of reflections	15624 *
Redundancy	6.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *		18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		30.0-32.5 (%sat)	or 5%(w/v) PEG8000 (incubation at 29 C)
2	1	reservoir	Bis-Tris-IDA	50 (mM)	or 50 mM Bis-Tris-HCl
3	1	reservoir	EDTA	0.7 (mM)	can be done without EDTA
4	1	reservoir	benzamidine	0.3 (mM)