1LI5
Crystal Structure of Cysteinyl-tRNA Synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-01-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 119.160, 119.160, 144.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.890 - 2.300 |
R-factor | 0.245 * |
Rwork | 0.245 |
R-free | 0.28300 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 2.010 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.069 | 0.307 |
Number of reflections | 45949 | |
<I/σ(I)> | 16.4 | 4.6 |
Completeness [%] | 98.8 | 99.8 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 17 * | 16% PEG 8000, 200 MM magnesium acetate, 1MM DTT, cacodylate, 5MM ATP, 10MM cysteine. 100 MM BUFFER, pH 6.5, VAPOR DIFFUSION, HANGING DROP at 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
10 | 1 | reservoir | magnesium acetate | 200 (mM) | |
11 | 1 | reservoir | t-butanol | 2 (%) | |
2 | 1 | drop | HEPES | 10 (mM) | pH7.4 |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | drop | 5 (mM) | ||
6 | 1 | drop | ATP | 5 (mM) | |
7 | 1 | drop | cysteine | 10 (mM) | |
8 | 1 | reservoir | PEG8000 | 15-17 (%) | |
9 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |