1LI1
The 1.9-A crystal structure of the noncollagenous (NC1) domain of human placenta collagen IV shows stabilization via a novel type of covalent Met-Lys cross-link
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | H 3 |
| Unit cell lengths | 234.589, 234.589, 99.492 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.950 - 1.900 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.20200 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 25.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.950 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 * | 0.300 * |
| Total number of observations | 441349 * | |
| Number of reflections | 155867 | |
| Completeness [%] | 96.9 | 97.6 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.1 | 293 | MES, sodium acetate, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | reservoir | MES-NaOH | 0.1 (M) | |
| 4 | 1 | reservoir | sodium acetate | 3.2-3.6 (M) | pH7.1-7.5 |
