1LHK

ROLE OF PROLINE RESIDUES IN HUMAN LYSOZYME STABILITY: A SCANNING CALORIMETRIC STUDY COMBINED WITH X-RAY STRUCTURE ANALYSIS OF PROLINE MUTANTS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	56.680, 61.040, 33.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.800
R-factor	0.153
RMSD bond length	0.016
RMSD bond angle	0.036 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.048 *
Total number of observations	39225 *
Number of reflections	10105 *
Completeness [%]	88.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.8 *	13 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1		2.5 (M)
3	1	1	sodium phosphate	30 (mM)