1LGR

INTERACTIONS OF NUCLEOTIDES WITH FULLY UNADENYLYLATED GLUTAMINE SYNTHETASE FROM SALMONELLA TYPHIMURIUM

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	235.500, 134.500, 200.100
Unit cell angles	90.00, 102.80, 90.00

Refinement procedure

Resolution	8.000 - 2.790
R-factor	0.233
Rwork	0.233
RMSD bond length	0.011
RMSD bond angle	3.000
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.066 *
Total number of observations	146242 *
Number of reflections	99219 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	imidazole hydrochloride	15 (mM)
2	1	drop		3 (mM)
3	1	drop	spermine tetrahydrochloride	3 (mM)
4	1	drop	MPD	10 (%)
5	1	reservoir	potassium phosphate-sodium hydroxide	0.05 (M)
6	1	reservoir	ammonium sulfate	2.95 (M)