1LGQ
Crystal structure of the FHA domain of the Chfr mitotic checkpoint protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Detector technology | CCD |
| Collection date | 2000-11-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.909 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.900, 52.900, 77.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.100 |
| R-factor | 0.242 * |
| Rwork | 0.242 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 26.400 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 * | |
| Total number of observations | 71014 * | |
| Number of reflections | 13548 | |
| Completeness [%] | 96.7 | 82.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | PEG 8000, Bis-Tris 6.5, DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277.16K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | bis-Tris | 20 (mM) | pH6.5 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 14-18 (%) | |
| 5 | 1 | reservoir | Bis-Tris | 100 (mM) | pH6.5 |
| 6 | 1 | reservoir | dithiothreitol | 5 (mM) |
