1LGH

CRYSTAL STRUCTURE OF THE LIGHT-HARVESTING COMPLEX II (B800-850) FROM RHODOSPIRILLUM MOLISCHIANUM

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1989-09-08
Detector	MARRESEARCH
Spacegroup name	P 4 21 2
Unit cell lengths	91.600, 91.600, 209.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.211
Rwork	0.211
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AS A SEARCH MODEL FOR MOLECULAR REPLACEMENT SERVED A COMPUTATIONALLY MODELLED OCTAMER OF ALPHA BETA-HETERODIMERS BASED ON A HOMOLOGY OF THE LATTER TO THE ALPHA BETA-HETERODIMER FROM RPS. ACIDOPHILA.
RMSD bond length	0.015
RMSD bond angle	21.814 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.400	2.400
Rmerge	0.097	0.311
Number of reflections	31941
Completeness [%]	89.1	66.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	LH-2	20 (mg/ml)
2	1	drop	HPTO	3.2 (%(w/v))
3	1	drop	ammonium sulfate	4 (M)
4	1	reservoir	ammonium sulfate	3.0-3.3 (M)