1LG7
Crystal structure of Vesicular Stomatitis Virus Matrix Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 200 |
Detector technology | IMAGE PLATE |
Collection date | 1999-09-17 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.00 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 80.690, 80.690, 51.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.960 |
R-factor | 0.206 * |
Rwork | 0.208 |
R-free | 0.24100 |
Structure solution method | SIRAS |
RMSD bond length | 0.005 * |
RMSD bond angle | 1.470 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.010 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.069 * | 0.410 * |
Total number of observations | 209741 * | |
Number of reflections | 12771 * | |
<I/σ(I)> | 30 | 3.5 |
Completeness [%] | 99.6 | 95.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | Gaudier, M., (2001) Virology, 288, 308. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 20 (mM) | pH8.5 |
2 | 1 | drop | 100 (mM) | ||
3 | 1 | drop | protein | 5 (mg/ml) | |
4 | 1 | reservoir | PEG2000 MME | 24 (%) | |
5 | 1 | reservoir | sodium acetate | 0.2 (M) | |
6 | 1 | reservoir | 0.1 (M) | ||
7 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |