1LFW
Crystal structure of pepV
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.151, 77.025, 89.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 * - 1.800 |
Rwork | 0.174 |
R-free | 0.20460 * |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.740 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8 * | 18 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
3 | 1 | drop | (L,D)AspPsi[PO2CH2](L,D)AlaOH | 3 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
5 | 1 | reservoir | 0.01 (M) | ||
6 | 1 | reservoir | MES- NaOH | 0.05 (M) | pH5.6 |
7 | 1 | reservoir | PEG8000 | 30 (%) |