1LFO
LIVER FATTY ACID BINDING PROTEIN-OLEATE COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Collection date | 1991-08-26 |
| Detector | SIEMENS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.890, 83.890, 44.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SUPERIMPOSED POLYALANINE COMPOSITE STRUCTURE OF 1ADL 1cbr 1crb 1opb 1ifc 1hmr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 27.700 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.800 | 2.300 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.100 * | 0.535 * |
| Number of reflections | 8640 | 940 * |
| <I/σ(I)> | 10.7 | 0.89 |
| Completeness [%] | 79.3 | 33.2 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 4 * | HANGING DROP VAPOR DIFFUSION EXPERIMENT 1 ML WELL: 3 M AMMONIUM SULFATE, 200 MM LISO4, 100 MM CITRATE, AT A PH OF 5.6 STOCK: 13 MG/ML LFABP-OLEATE COMPLEX 10 MICROLITER DROP: 1:1 MIXTURE OF STOCK AND WELL, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 3 (M) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | citrate | 100 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 1.5 (M) | |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | citrate | 50 (mM) | |
| 7 | 1 | drop | protein | 6.5 (mg/ml) |
