1LF9
CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE COMPLEXED WITH ACARBOSE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.491, 102.931, 164.686 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.200 |
Rwork | 0.191 |
R-free | 0.23000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lf6 |
RMSD bond length | 0.006 |
RMSD bond angle | 22.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.110 | 0.400 |
Total number of observations | 268721 * | |
Number of reflections | 65095 | |
<I/σ(I)> | 14 | |
Completeness [%] | 92.0 | 83 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 24 * | PEG 3350, Tris-HCl, Lithium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
2 | 1 | reservoir | PEG3350 | 14-16 (%) | |
3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.0 |
4 | 1 | reservoir | 200 (mM) |