1LF1

Crystal Structure of Cel5 from Alkalophilic Bacillus sp.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	70.440, 78.160, 55.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.700
R-factor	0.1895
Rwork	0.186
R-free	0.21000 *
RMSD bond length	0.014 *
RMSD bond angle	1.790 *
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.000
High resolution limit [Å]	1.700	1.700
Rmerge	0.077 *
Total number of observations	139842 *
Number of reflections	32428
<I/σ(I)>	12.4	3.61
Completeness [%]	97.7	92.4
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.5	295	Naki, D., (1998) Appl. Microb. Biotechnol., 49, 290. *