1LF0
Crystal Structure of RasA59G in the GTP-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 89.129, 89.129, 134.654 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 * - 1.700 |
Rwork | 0.185 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Wild-type Ras |
RMSD bond length | 0.009 |
RMSD bond angle | 23.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.046 | 0.108 |
Total number of observations | 255064 * | |
Number of reflections | 21166 * | |
<I/σ(I)> | 2 | 2 |
Completeness [%] | 92.7 | 86.4 |
Redundancy | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 295 | PEG1500, calcium chloride, magnesium chloride, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | drop | HEPES | 20 (mM) | |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | 10 (mM) | pH7.5 | |
5 | 1 | reservoir | PEG1500 | 20 (%(w/v)) | |
6 | 1 | reservoir | 100 (mM) | ||
7 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.5 |