1LEM

THE MONOSACCHARIDE BINDING SITE OF LENTIL LECTIN: AN X-RAY AND MOLECULAR MODELLING STUDY

Experimental procedure

Spacegroup name	P 65 2 2
Unit cell lengths	85.710, 85.710, 165.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 3.000
R-factor	0.206
Rwork	0.206
RMSD bond length	0.018
RMSD bond angle	3.200 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	3.000 *
Rmerge	0.070 *
Total number of observations	35751 *
Number of reflections	6950 *
Completeness [%]	94.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	lentil lectin	10 (mg/ml)
2	1	drop	cacodylate	100 (mM)
3	1	drop	glucose	50 (mM)
4	1	drop	ethanol	15 (%)
5	1	reservoir	ethanol	35 (%)
6	1	reservoir	cacodylate	100 (mM)