1LEH

LEUCINE DEHYDROGENASE FROM BACILLUS SPHAERICUS

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	I 4
Unit cell lengths	138.400, 138.400, 121.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
Rwork	0.201
RMSD bond length	0.012
RMSD bond angle	2.095
Data reduction software	MOSFLM
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	19.800
High resolution limit [Å]	2.200
Rmerge	0.053
Total number of observations	190002 *
Number of reflections	57499 *
Completeness [%]	99.8
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5 (mg/ml)
2	1	drop	MES-NaOH	0.1 (M)
3	1	drop	EDTA	1 (mM)
4	1	reservoir	ammonium sulfate	1.8-2.1 (M)
5	1	reservoir	MES-NaOH	0.1 (M)
6	1	reservoir	EDTA	1 (mM)