1LEH
LEUCINE DEHYDROGENASE FROM BACILLUS SPHAERICUS
Experimental procedure
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.5 |
Synchrotron site | SRS |
Beamline | PX9.5 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | I 4 |
Unit cell lengths | 138.400, 138.400, 121.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.200 |
Rwork | 0.201 |
RMSD bond length | 0.012 |
RMSD bond angle | 2.095 |
Data reduction software | MOSFLM |
Refinement software | TNT |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.800 |
High resolution limit [Å] | 2.200 |
Rmerge | 0.053 |
Total number of observations | 190002 * |
Number of reflections | 57499 * |
Completeness [%] | 99.8 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 2.5 (mg/ml) | |
2 | 1 | drop | MES-NaOH | 0.1 (M) | |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 1.8-2.1 (M) | |
5 | 1 | reservoir | MES-NaOH | 0.1 (M) | |
6 | 1 | reservoir | EDTA | 1 (mM) |