1LE8

Crystal Structure of the MATa1/MATalpha2-3A Heterodimer Bound to DNA Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	98
Detector technology	CCD
Collection date	1999-05-05
Detector	KODAK
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	54.170, 54.170, 164.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.280 - 2.300
R-factor	0.255 *
Rwork	0.256
R-free	0.29800 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007 *
RMSD bond angle	1.180 *
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300	2.300
Rmerge	0.030 *	0.294 *
Total number of observations	48164 *
Number of reflections	11201
<I/σ(I)>	19.6	3
Completeness [%]	92.5	53.2
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	10% PEG400, 8 mM Co(NH3)6Cl3, 10 mM CaCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG400
2	1	1	Co(NH3)6Cl3
3	1	1	CaCl2