1LE4

STRUCTURAL BASIS FOR ALTERED FUNCTION IN THE COMMON MUTANTS OF HUMAN APOLIPOPROTEIN-E

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	40.710, 53.330, 85.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.500
Rwork	0.175
RMSD bond length	0.015
RMSD bond angle	3.100
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Number of reflections	6752 *
Completeness [%]	99.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservior	sodium acetate	20 (mM)
3	1	reservoir	beta-n-glycopyranoside	0.2 (%)
4	1	reservoir	PEG400	15 (%)