1LDT
COMPLEX OF LEECH-DERIVED TRYPTASE INHIBITOR WITH PORCINE TRYPSIN
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1995-01 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 63.400, 63.400, 131.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.900 |
R-factor | 0.197 |
Rwork | 0.197 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PORCINE TRYPSIN MODEL FROM TRYPSIN:MUNG BEAN INHIBITOR (LIN ET AL. EUR. J. BIOCHEM. 212 549-555 (1993) |
RMSD bond length | 0.008 |
RMSD bond angle | 24.600 * |
Data reduction software | MOSFLM |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.082 | 0.200 |
Total number of observations | 139563 * | |
Number of reflections | 21466 | |
<I/σ(I)> | 9 | 4 |
Completeness [%] | 98.6 | 87.6 |
Redundancy | 6.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 | 10% PEG 6000, 2.3M PHOSPHATE, PH 8.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Mops | 20 (mM) | |
3 | 1 | drop | 20 (mM) | ||
4 | 1 | reservoir | PEG6000 | 10 (%) | |
5 | 1 | reservoir | phosphate | 2.3 (M) |