1LDP
CRYSTAL STRUCTURE OF MURINE MHC CLASS I H-2LD WITH A MIXTURE OF BOUND PEPTIDES
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-18 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-01 |
| Detector | SIEMENS |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 72.400, 48.980, 84.060 |
| Unit cell angles | 90.00, 97.95, 90.00 |
Refinement procedure
| Resolution | 6.000 - 3.100 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.37100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hoc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 25.800 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.825) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.113 | 0.358 |
| Number of reflections | 11809 | 926 * |
| <I/σ(I)> | 5.6 | 1.8 |
| Completeness [%] | 81.0 | 63.6 |
| Redundancy | 2.2 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | PROTEIN WAS CRYSTALLIZED FROM 100MM SODIUM CACODYLATE, 0.2M AMMONIUM SULFATE, 0.5M SODIUM BROMIDE, 30% PEG 8000, PH 6.5. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | peptide | 7 (mg/ml) | |
| 2 | 1 | 1 | sodium cacodylate | 100 (mM) | |
| 3 | 1 | 1 | ammonium sulfate | 0.2 (M) | |
| 4 | 1 | 1 | 0.5 (M) | ||
| 5 | 1 | 1 | PEG8000 | 30 (%) |
