1LDO
avidin-norbioitn complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 2001-12-10 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 71.103, 79.813, 43.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
Rwork | 0.183 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.115 * | 0.240 * |
Number of reflections | 12800 | |
Completeness [%] | 97.4 | 89 |
Redundancy | 2. * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 20 * | 12% PEG 1000, 0.1 immidazole malate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.2 (mg/ml) | |
2 | 1 | reservoir | PEG1000 | 12 (%) | |
3 | 1 | reservoir | imidazole-malate | 0.1 (M) | pH5.8 |