1LDG

PLASMODIUM FALCIPARUM L-LACTATE DEHYDROGENASE COMPLEXED WITH NADH AND OXAMATE

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-05-29
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	80.200, 86.800, 90.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.740
R-factor	0.197
Rwork	0.197
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010 *
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.740
Rmerge	0.066
Number of reflections	32471
Completeness [%]	99.6
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	26 (mg/ml)
2	1	drop	NADH	0.025 (M)
3	1	drop	sodium oxamate	10 (mM)
4	1	reservoir	MPD	30 (%(v/v))
5	1	reservoir	sodium citrate	200 (mM)
6	1	reservoir	HEPES	100 (mM)