1LDD

Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF Ubiquitin Ligase Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	170
Detector technology	CCD
Wavelength(s)	0.9747
Spacegroup name	P 21 21 21
Unit cell lengths	54.700, 72.900, 79.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.205 *
Rwork	0.205
R-free	0.26600
Structure solution method	MAD
RMSD bond length	0.012 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000
Rmerge	0.052 *
Total number of observations	120747 *
Number of reflections	40867
Completeness [%]	97.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	4 *	PEG4K, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	80 (mg/ml)
2	1	reservoir	PEG4000	18.5 (%)
3	1	reservoir		0.16 (M)
4	1	reservoir	Tris	0.1 (M)	pH8.5