1LD9
THE THREE-DIMENSIONAL STRUCTURE OF AN H-2LD PEPTIDE COMPLEX EXPLAINS THE UNIQUE INTERACTION OF LD WITH BETA2M AND PEPTIDE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-08-27 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 150.100, 87.200, 80.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.400 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hoc |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 2.100 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.130 |
| Number of reflections | 28578 | |
| <I/σ(I)> | 3.7 | 3.7 |
| Completeness [%] | 67.0 | 58 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 5 (mg/ml) | |
| 2 | 1 | drop | 0.7 (M) | ||
| 3 | 1 | drop | glycerol | 1-4 (%) | |
| 4 | 1 | reservoir | 1.4 (M) | ||
| 5 | 1 | reservoir | glycerol | 2-8 (%) |
