1LCV
streptavidin-norbiotin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01-01 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 47.290, 95.320, 105.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
Rwork | 0.188 |
R-free | 0.23400 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.260 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.094 * | 0.353 * |
Number of reflections | 10095 | |
Completeness [%] | 92.2 | 93.4 |
Redundancy | 2. * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 * | 20 * | 1.2 M ammonium sulfate, 0.1M sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 4.2 (mg/ml) | |
2 | 1 | reservoir | PEG1000 | 12 (%) | |
3 | 1 | reservoir | imidazole-malate | 0.1 (M) | pH5.8 |