1LCT
STRUCTURE OF THE RECOMBINANT N-TERMINAL LOBE OF HUMAN LACTOFERRIN AT 2.0 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.000, 58.300, 58.300 |
| Unit cell angles | 90.00, 114.70, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.184 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 3.000 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.075 * |
| Number of reflections | 35000 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.8 * | 4 * | taken from Day, C.L. et al(1992). J. Mol. Biol., 228, 973-974. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 0.1 (M) | ||
| 2 | 1 | 1 | 0.2 (M) | ||
| 3 | 1 | 1 | HEPES | 0.01 (M) | |
| 4 | 1 | 1 | ethanol | or 12 % isopropanol |
