1LCN
Monoclinic hen egg white lysozyme, thiocyanate complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 1998-09-15 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.010, 63.180, 60.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.830 - 1.630 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MONOMER A FROM 5LYM PDB ENTRY (MONOCLINIC LYSOZYME) |
RMSD bond length | 0.006 |
RMSD bond angle | 1.350 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.830 | 1.690 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.028 * | |
Number of reflections | 25539 | |
<I/σ(I)> | 15.2 | 6.3 |
Completeness [%] | 96.8 | 89.5 |
Redundancy | 4.2 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 * | 291 * | KSCN 190MM BUFFER CH3COOK 50MM, PH=5, pH 5.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.7 (mg/ml) | |
2 | 1 | drop | potassium acetate | 50 (mM) | |
3 | 1 | drop | 190 (mM) |