1LCL

CHARCOT-LEYDEN CRYSTAL PROTEIN

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	289
Detector technology	IMAGE PLATE
Collection date	1994
Detector	MARRESEARCH
Wavelength(s)	0.8, 1.0
Spacegroup name	P 65 2 2
Unit cell lengths	49.600, 49.600, 262.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.2
Rwork	0.200
R-free	0.25800
RMSD bond length	0.010
RMSD bond angle	1.610
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.800
Rmerge	0.077
Total number of observations	75363 *
Number of reflections	18046
Completeness [%]	94.4
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	10.5 *	16 *	pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris	0.1 (M)
3	1	reservoir	Tris-acetate	0.2 (M)