1LC5

Crystal Structure of L-Threonine-O-3-phosphate Decarboxylase from S. enterica in its apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	0.9763
Spacegroup name	I 2 2 2
Unit cell lengths	75.990, 103.340, 109.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 1.460
Rwork	0.190
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1KUS
RMSD bond length	0.011
RMSD bond angle	1.540
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	1.510
High resolution limit [Å]	1.460	1.460
Rmerge	0.040	0.129
Number of reflections	74541
<I/σ(I)>	66.7	13.2
Completeness [%]	99.4	95.4
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	sodium potassium phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP at 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	sodium potassium phosphate	1.1 (M)
3	1	reservoir	lithium sulfate	50 (mM)	pH6.0