1LBW
Crystal Structure of apo-form (P32) of dual activity FBPase/IMPase (AF2372) from Archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-11-02 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 |
Unit cell lengths | 89.368, 89.368, 103.558 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.177 * |
Rwork | 0.186 |
R-free | 0.21200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dk4 |
RMSD bond length | 0.008 * |
RMSD bond angle | 0.026 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.040 | 0.410 |
Number of reflections | 55200 * | |
<I/σ(I)> | 28.1 | 2.7 |
Completeness [%] | 92.4 * | 90.5 * |
Redundancy | 3.0 * | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 3350, ammonium nitrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium nitrate | 0.2 (M) | |
2 | 1 | reservoir | PEG3350 | 30 (%) | |
3 | 1 | drop | protein | 10 (mg/ml) |