1LBV
Crystal Structure of apo-form (P21) of dual activity FBPase/IMPase (AF2372) from Archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-07-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.009 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.812, 53.247, 82.154 |
Unit cell angles | 90.00, 105.05, 90.00 |
Refinement procedure
Resolution | 31.810 - 1.800 |
R-factor | 0.203 * |
Rwork | 0.203 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dk4 |
RMSD bond length | 0.007 * |
RMSD bond angle | 0.020 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.036 | 0.270 * |
Number of reflections | 35243 * | |
<I/σ(I)> | 16.8 | 3.2 |
Completeness [%] | 93.7 * | 83.9 * |
Redundancy | 3.6 * | 2.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 3350, ammonium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | dihydrogen ammonium phosphate | 0.2 (M) | |
2 | 1 | reservoir | PEG3350 | 30 (%) | |
3 | 1 | drop | protein | 10 (mg/ml) |