1LBT
LIPASE (E.C.3.1.1.3) (TRIACYLGLYCEROL HYDROLASE)
Experimental procedure
Detector technology | AREA DETECTOR |
Collection date | 1990-06-18 |
Detector | XUONG-HAMLIN MULTIWIRE MARK III |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.100, 50.200, 99.500 |
Unit cell angles | 90.00, 90.60, 90.00 |
Refinement procedure
Resolution | 7.500 - 2.500 |
R-factor | 0.172 |
Rwork | 0.172 |
R-free | 0.19300 |
RMSD bond length | 0.007 |
RMSD bond angle | 23.800 * |
Data reduction software | MARK (III) |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.500 | 2.500 * |
Rmerge | 0.041 | 0.123 * |
Total number of observations | 45529 * | |
Number of reflections | 26677 | |
Completeness [%] | 82.0 * | 63 * |
Redundancy | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 20-25 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
2 | 1 | reservoir | dioxane | 10 (%) | |
3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | drop | beta-octylglucoside | 4 (%(w/v)) | |
6 | 1 | drop | ammonium sulfate | 1.0 (1.0) | |
7 | 1 | drop | dioxane | 10 (%) | |
8 | 1 | drop | sodium citrate | 0.1 (M) |