1LBT
LIPASE (E.C.3.1.1.3) (TRIACYLGLYCEROL HYDROLASE)
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1990-06-18 |
| Detector | XUONG-HAMLIN MULTIWIRE MARK III |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.100, 50.200, 99.500 |
| Unit cell angles | 90.00, 90.60, 90.00 |
Refinement procedure
| Resolution | 7.500 - 2.500 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.19300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.800 * |
| Data reduction software | MARK (III) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.500 | 2.500 * |
| Rmerge | 0.041 | 0.123 * |
| Total number of observations | 45529 * | |
| Number of reflections | 26677 | |
| Completeness [%] | 82.0 * | 63 * |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 20-25 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
| 2 | 1 | reservoir | dioxane | 10 (%) | |
| 3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | beta-octylglucoside | 4 (%(w/v)) | |
| 6 | 1 | drop | ammonium sulfate | 1.0 (1.0) | |
| 7 | 1 | drop | dioxane | 10 (%) | |
| 8 | 1 | drop | sodium citrate | 0.1 (M) |
