1LBQ

The crystal structure of Saccharomyces cerevisiae ferrochelatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-11
Detector	MARRESEARCH
Wavelength(s)	0.96776
Spacegroup name	P 21 21 21
Unit cell lengths	86.610, 97.169, 121.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.400
Rwork	0.255
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	human ferrochelatase pdb code: 1hrk
RMSD bond length	0.007 *
RMSD bond angle	1.600 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.105	0.199 *
Number of reflections	37088 *
Completeness [%]	92.5	80.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	4 *	Peg2000, 2-propanol, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	0.1 (M)	pH8.0
2	1	drop	glycerol	20 (%(w/v))
3	1	drop	n-octyl-beta-D-glucopyranoside	1 (%(w/v))
4	1	reservoir	Tris-HCl	0.1 (M)	pH7.5
5	1	reservoir	PEG2000	23 (%(w/v))
6	1	reservoir	2-propanol	10 (%(v/v))