1LBM

CRYSTAL STRUCTURE OF PHOSPHORIBOSYL ANTHRANILATE ISOMERASE (PRAI) IN COMPLEX WITH REDUCED 1-(O-CARBOXYPHENYLAMINO)-1-DEOXYRIBULOSE 5-PHOSPHATE (RCDRP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-07-25
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 61 2 2
Unit cell lengths	68.835, 68.834, 187.163
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
Rwork	0.170
R-free	0.22800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1nsj
RMSD bond length	0.010
RMSD bond angle	1.800
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Rmerge	0.099
Total number of observations	28569 *
Number of reflections	6978
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	277	50 mM potassium phosphate , 2.1 M ammonium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	19 (mg/ml)
2	1	reservoir	MES	0.1 (M)	pH7.0
3	1	reservoir	ammonium sulfate	1.5 (M)