1LBE

APLYSIA ADP RIBOSYL CYCLASE

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	70.200, 72.300, 125.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.214
Rwork	0.214
R-free	0.29900
RMSD bond length	0.017
RMSD bond angle	2.400
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.103
Total number of observations	84127 *
Number of reflections	25029 *
Completeness [%]	98.3
Redundancy	3.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	18 *	drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8.0 (OD280)
2	1	drop		40 (mM)
3	1	drop	HEPES	10 (mM)
4	1	reservoir	PEG4000	30 (%(w/v))
5	1	reservoir	imidazole-malate	0.2 (M)