1LAJ
The Structure of Tomato Aspermy Virus by X-Ray Crystallography
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1997-04-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.15000 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 294.296, 327.393, 382.066 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.400 |
Rwork | 0.218 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f15 |
RMSD bond length | 0.009 |
RMSD bond angle | 29.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.720 | 3.460 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.230 | |
Total number of observations | 1444765 * | |
Number of reflections | 156468 | |
<I/σ(I)> | 7.9 | |
Completeness [%] | 62.0 * | 2.2 |
Redundancy | 1.522 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 18 * | 14-17% Ethanol, 100mM tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 18 * | 14-17% Ethanol, 100mM tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 14 (mg/ml) | pH8.5 |
2 | 1 | drop | ethanol | 14-17 (%) | |
3 | 1 | drop | Tris | 100 (mM) | |
4 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
5 | 1 | reservoir | MPD | 20 (%) | |
6 | 1 | reservoir | ethanol | 10 (%) | |
7 | 1 | reservoir | sodium azide | 0.04 (%) |