1LA2
Structural analysis of Saccharomyces cerevisiae myo-inositol phosphate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 155.693, 187.346, 98.954 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.650 |
| R-factor | 0.224 * |
| Rwork | 0.224 |
| R-free | 0.28000 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 * |
| RMSD bond angle | 1.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.105 | 0.448 |
| Total number of observations | 1002153 * | |
| Number of reflections | 180676 | |
| <I/σ(I)> | 10.9 | 4.3 |
| Completeness [%] | 98.6 | 91 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 291 | 7% PEG 4000, 0.1M sodium acetate, 5% glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.4 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH8. |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 7 (%) | |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 7 | 1 | reservoir | glycerol | 5 (%) |
