1LA1
Gro-EL Fragment (Apical Domain) Comprising Residues 188-379
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.620, 61.950, 75.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.500 - 2.060 |
| R-factor | 0.197 * |
| Rwork | 0.197 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Apical domain derived from 1OEL |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.400 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.060 |
| Rmerge | 0.387 * |
| Number of reflections | 14319 |
| <I/σ(I)> | 10.1 |
| Completeness [%] | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 18 * | 0.6 M NaCl, 14 % (w/v) PEG 6000, 100 mM Tris/HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | pH7.2 |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | reservoir | 0.6 (M) | ||
| 5 | 1 | reservoir | PEG6000 | 14 (%(w/v)) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.5 |
