1L9Z
Thermus aquaticus RNA Polymerase Holoenzyme/Fork-Junction Promoter DNA Complex at 6.5 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.94799 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 180.947, 180.947, 523.461 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.000 - 6.500 |
R-factor | 0.448 * |
R-free | 0.45200 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 6.730 |
High resolution limit [Å] | 6.500 | 6.500 |
Rmerge | 0.085 | 0.241 * |
Number of reflections | 16195 | |
<I/σ(I)> | 11.5 | |
Completeness [%] | 90.9 | 78.5 |
Redundancy | 5.7 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | ammonium sulfate, Tris, Magnesium chloride, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ammonium sulfate | ||
2 | 1 | 1 | Tris | ||
3 | 1 | 1 | MgCl2 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 1.6-1.8 (M) | |
3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
4 | 1 | reservoir | 20 (mM) |