1L9Y
FEZ-1-Y228A, A Mutant of the Metallo-beta-lactamase from Legionella gormanii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.761, 77.430, 78.499 |
| Unit cell angles | 90.00, 101.70, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.010 |
| Rwork | 0.186 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k07 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.233 | 2.120 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.073 | 0.144 |
| Number of reflections | 33715 | |
| <I/σ(I)> | 8.3 | 6 |
| Completeness [%] | 97.0 | 88.2 |
| Redundancy | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281.15 | 20%PEG MME 5000, 0.2M amm.sulfate, 0.1M Cacod.acid/Na Cacodylate, 10microM Zn acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 281.15K |
