1L9L
GRANULYSIN FROM HUMAN CYTOLYTIC T LYMPHOCYTES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-10 |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.265, 60.436, 23.591 |
| Unit cell angles | 90.00, 90.99, 90.00 |
Refinement procedure
| Resolution | 23.590 - 0.920 |
| R-factor | 0.138 |
| Rwork | 0.137 |
| R-free | 0.18980 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.038 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.600 | 0.960 |
| High resolution limit [Å] | 0.920 | 0.920 |
| Rmerge | 0.037 | 0.300 * |
| Number of reflections | 35664 | |
| <I/σ(I)> | 48.3 | 3.8 |
| Completeness [%] | 82.3 | 31.7 |
| Redundancy | 7.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 21-22 * | 3.1-3.2 M AMMONIUM SULFATE, 0.05 M N-MORPHOLINO PROPANESULFONIC ACID TITRATED TO PH 7 WITH SODIUM HYDROXIDE, 2.5% ETHANOL., VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 3.1-3.2 (M) | |
| 3 | 1 | reservoir | sodium MOPS | 0.05 (M) | pH7. |
| 4 | 1 | reservoir | ethanol | 2.5 (%(v/v)) | |
| 5 | 1 | drop | ammonium sulfate | 3.2 (M) | |
| 6 | 1 | drop | sodium MOPS | 0.05 (M) | |
| 7 | 1 | drop | ethanol | 2.5 (%(v/v)) |
