1L9A
CRYSTAL STRUCTURE OF SRP19 IN COMPLEX WITH THE S DOMAIN OF SIGNAL RECOGNITION PARTICLE RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-30 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9185 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.765, 223.875, 43.024 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.840 - 2.900 |
R-factor | 0.264 |
Rwork | 0.264 |
R-free | 0.29600 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 17.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.081 | 0.310 |
Number of reflections | 26760 | |
<I/σ(I)> | 18.7 | 3.1 |
Completeness [%] | 91.4 | 52.9 |
Redundancy | 6.4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 27.5 * | 12% PEG4000, 110MM Tris.HCl pH 7.4, 10% glycerol, 50mm magnesium acetate, 100MM ammonium acetate, 5MM magnesium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 300.5K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | ammonium acetate | 100 (mM) | |
3 | 1 | drop | Tris-HCl | 10 (mM) | pH7.4 |
4 | 1 | drop | glycerol | 10 (%) | |
5 | 1 | drop | 5 (mM) | ||
6 | 1 | drop | dithiothreitol | 5 (mM) | |
7 | 1 | reservoir | PEG4000 | 12 (%(w/v)) | |
8 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.4 |
9 | 1 | reservoir | magnesium acetate | 50 (mM) |