1L8V
Crystal Structure of a Mutant (C109G,G212C) P4-P6 Domain of the Group I Intron from Tetrahymena Thermophilia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-04-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.455, 129.918, 144.686 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 99.000 * - 2.800 |
R-factor | 0.29 |
Rwork | 0.290 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gid |
RMSD bond length | 0.006 |
RMSD bond angle | 15.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.980 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.051 * | 0.160 * |
Number of reflections | 33255 | |
<I/σ(I)> | 32.9 | |
Completeness [%] | 87.3 | 95.2 * |
Redundancy | 7.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 * | 25 * | MPD, magnesium chloride, cacodylate, spermine, sodium chloride, pH 6.5, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | MgCl | ||
3 | 1 | 1 | cacodylate | ||
4 | 1 | 1 | spermine | ||
5 | 1 | 1 | NaCl |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 22 (%) | ||
2 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.5 |
3 | 1 | reservoir | spermine | 0.05 (mM) | |
4 | 1 | drop | protein | 4 (mg/ml) | |
5 | 1 | drop | HEPES-NaOH | 5 (mM) | pH7.5 |
6 | 1 | drop | 5 (mM) | ||
7 | 1 | drop | 40-70 (mM) |