1L8R

Structure of the Retinal Determination Protein Dachshund Reveals a DNA-Binding Motif

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-01
Detector	CUSTOM-MADE
Wavelength(s)	0.97981, 0.97904, 0.95366
Spacegroup name	P 1
Unit cell lengths	28.797, 47.731, 50.436
Unit cell angles	60.75, 86.86, 84.55

Refinement procedure

Resolution	6.000 * - 1.650
R-factor	0.237
Rwork	0.237
R-free	0.25100 *
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.120
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.064 *	0.233 *
Total number of observations	227661 *
Number of reflections	26123 *
Completeness [%]	96.5	91.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	277	PEG 10000, Ammonium Sulphate, pH 7.5, VAPOR DIFFUSION, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG10000	25-30 (%)
2	1	reservoir	ammonium sulfate	0.25 (M)
3	1	reservoir	dithiothreitol	5 (mM)
4	1	drop	protein	8 (mg/ml)