1L8N
The 1.5A crystal structure of alpha-D-glucuronidase from Bacillus stearothermophilus T-1, complexed with 4-O-methyl-glucuronic acid and xylotriose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2001-07-12 |
Detector | BRANDEIS |
Wavelength(s) | 1.1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 73.946, 73.946, 331.341 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.500 |
Rwork | 0.140 |
R-free | 0.17210 |
Structure solution method | AB INITIO |
Starting model (for MR) | pdb code 1k9d |
RMSD bond length | 0.012 |
RMSD bond angle | 2.130 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.110 | 0.399 |
Number of reflections | 145976 | |
<I/σ(I)> | 6.8 | |
Completeness [%] | 98.8 | 90.3 |
Redundancy | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1M NA-CITRATE PH 5.0, 14% PEG 4000, 12% ISO-PROPANOL, VAPOR DIFFUSION, HANGING DROP, temperature 293K |