1L8J

Crystal Structure of the Endothelial Protein C Receptor and Bound Phospholipid Molecule

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	110
Detector technology	CCD
Collection date	2001-03-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.03
Spacegroup name	P 31 2 1
Unit cell lengths	69.710, 69.710, 96.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 2.000
Rwork	0.195
R-free	0.21100
Structure solution method	MIR
RMSD bond length	0.012
RMSD bond angle	1.550
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.038	0.257
Number of reflections	18663
<I/σ(I)>	40	5
Completeness [%]	99.6	99.6
Redundancy	5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Sodium Formate, TRIS-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Sodium Formate, TRIS-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Sodium Formate, TRIS-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K